ALPHA CHEMIKA
Auramine for Microscopy

Auramine for Microscopy

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Auramine is known to be diarylmethane dye which acts as a version of Schiff reagent and its crystals appear to be yellow in color in their pure form. It is soluble in water and ethanol. Being a fluorescent stain in nature, our offered Auramine plays a crucial role in the process of microscopy with its staining capabilities of detecting acid-fast bacteria called Mycobaterium. During microscopy, possibility of artifacts can be minimized by the counter stain potassium permanganate. Thus, the cells viewed appear to be reddish orange or bright yellow in color under ultraviolet light. When it combines with Rhodamine, mycolic acids present in cell wall of various organisms helps in providing resistance against de-colorization with the help of acid-alcohol.

 

Certificate of Analysis :-

  • Name Of Item : AURAMINE (M.S.) (C.I. No. 41000)
  • Formula : C17H22N3Cl
  • M.W. : 303.84
  • Batch No. :  A0580
  • Cat. No. :  AL0511 00100


Technical Specifications :-

 

Type Of Test Standard Observed
Description Yellow colored powder Yellow colored powder
Absorption Max. 431-433nm 432 nm
Absorptivity ( A1%;    
1cm=432 nm , pH 6.0 on    
dried substance) 880 - 940 931.6
Loss on drying ( 105°C ) < 2% 1.2%
Suitability for microscopy Passes test Passes test


Results :
The  above product complies with  LR  grade