ALPHA CHEMIKA
COOMASIE BRILLIANT

COOMASIE BRILLIANT

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Coomassie Brilliant is similar to the name of triphenymethane dye that was introduced to find usage in the textile industry. In present time, it finds usage to stain and dye proteins during the experiments of anyalytical biochemistry. Color of the dyes that fall under Coomassie Brilliant depends upon the acidity possessed by the solution. Being non-covalent in nature, the mentioned dye interacts electrostatically with the proteins, carboxyl groups and amino. Moreover, it forms a complex structure when combined with sodium dodecylsulfate.

Certificate of Analysis :-

  • Name : COOMASIE BRILLIANT BLUE R 250
  • Synonyms : Acid Blue 83, Brilliant indocyanine 6B, Brilliant Cyanine 6B, Serva Blue R
  • Chemical Formula : C45H44N3NaO7S2
  • Molar Mass : 825.97 g/mol
  • PubChem CID : 61365
  • Beistein Registry Number : 5718025
  • EC Number : 228-060-5
  • Absorption : 589.2 nm
  • C.I. : 42660
  • pH : 6.2
  • HS Code : 3204-12-00
  • M.P. 174 – 180 deg C
  • Grade : For Microscopy
  • Solubility : 20 g/l
  • Loss on drying max. : 5%
  • Suitability for : Electrophoresis passes test
  • Un No. NR TARIFF : 3204 90 00
  • S : 22-24/25

Applications :-

During western blot analysis, Coomassie finds application within a loading control staining method to stain the proteins. Rats that are used in the laboratories to perform experiments, Coomassie helps in treating their spinal injuries.