Initially, Tetrahydrofuran was used for High-performance liquid chromatography as a mobile phase solvent. Its strength is similar to that of acetonitrile, infact, slightly higher than it. As it is an ether, it is easily miscible in water, finding application in the reverse phase of HPLC. This compound renders additional options to have ternary mixes, during incapability of combination of water and methanol or water with acetonitrile for producing a fine tuned separation. Moreover, in analytical chemistry it finds application for separating, quantifying and identifying each component in a particular mixture.
Technical Specifications of Tetrahydrofuran for HPLC & Spectroscopy